Ether-lipid membrane engineering of Escherichia coli

Ether-lipid membrane engineering of Escherichia coli

University of Groningen Ether-lipid membrane engineering of Escherichia coli Caforio, Antonella IMPORTANT NOTE: You are advised to consult the publi...

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University of Groningen

Ether-lipid membrane engineering of Escherichia coli Caforio, Antonella

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Document Version Publisher's PDF, also known as Version of record

Publication date: 2017 Link to publication in University of Groningen/UMCG research database

Citation for published version (APA): Caforio, A. (2017). Ether-lipid membrane engineering of Escherichia coli [Groningen]: University of Groningen

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Download date: 27-12-2017

Ether-lipid membrane engineering of Escherichia coli

Antonella Caforio

Cover design and layout of the book: Antonella Caforio Cover front and back: artistic interpretation of lipid rafts in a sea landscape. The lipid rafts are composed by two different lipid species representing archaeal and bacterial membrane lipds with the CarS structure embedded.

The research described in this thesis was carried out in the Department of Molecular Microbiology of the Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, The Netherlands. It was financially supported by the biobased ecologically balanced sustainable industrial chemistry (BE-BASIC). ISBN: 978-90-367-9379-7 ISBN: 978-90-367-9378-0 (electronic version) Printed by Ipskamp printing, Enschede Printing of this thesis was supported by generous contribution from the University of Groningen and the Groningen Biomolecular Sciences and Biotechnology Institute (GBB). Copyright © 2016 by Antonella Caforio. All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without the prior permission of the author.

Ether-lipid membrane engineering of Escherichia coli PhD thesis

to obtain the degree of PhD at the University of Groningen on the authority of the Rector Magnificus Prof. E. Sterken and in accordance with the decision by the College of Deans. This thesis will be defended in public on Friday 13 January 2017 at 14.30 hours by

Antonella Caforio born on 14 April 1988 in Francavilla Fontana, Italy

Supervisor Prof. A.J.M. Driessen

Assessment Committee Prof. D.J. Slotboom Prof. O.P. Kuipers Prof. S. Schouten

A mio padre, mia madre e mia sorella per avermi sempre sostenuto, incoraggiato e creduto in me dall’inizio alla fine. Al mio amore, per avermi donato amore e felicitá.

Table of Contents Chapter 1 Biosynthesis of archaeal membrane ether lipids….......9

Chapter 2 Identification of CDP-archaeol synthase, a missing link of ether lipid biosynthesis in Archaea……………………………………………...……...……..41

Chapter 3 Formation of the ether lipids archaeatidylglycerol and archaeatidylethanolamine in Escherichia coli……………………………95

Chapter 4 Engineering of Escherichia coli with a hybrid heterochiral membrane………………………………………129

Chapter 5 Structural insights into lipid biosynthesis in archaeal membranes…………………………………………..185

Chapter 6 Saturation of the ether lipids double bonds: Geranylgeranyl reductase…………………………………...217

Chapter 7 Summary……………………………………………………………231