Prodigy (Phenomenex)

Prodigy (Phenomenex)

PRODIGY™ If Prodigy does not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and ...

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GC Application: ASTM Method D5060 - Phenomenex
ASTM Method D5060: Determining Impurities in High Purity Ethylbenzene on ZB-FFAP. Column: Phase: Dimensions: Order No: O

PRODIGY™ If Prodigy does not provide at least an equivalent separation as compared to a competing column of the same particle size, similar phase and dimensions, send in your comparative data within 45 days and keep the Prodigy column for FREE.

• PH-3: phenyl column with excellent aromatic and polar compound selectivity • 3 μm ODS-3: high efficiency alternative to the 5 μm ODS-3 columns • Columns stable from pH 2 to 9

Prodigy

HPLC

Material Characteristics Particle Pore Pore Surface Carbon Phase Shape/Size Size Volume Area Load 2 % (μm) (Å) (mL/g) (m /g) Silica Spher. 5 150 — 310 0 ODS (2) Spher. 5 150 1.1 310 18.5, Monomeric C8 Spher. 5 150 1.1 310 12.6, Monomeric ODS (3) Spher. 3, 5, 10 100 — 450 15.5, Monomeric Phenyl-3 (PH-3) Spher. 5 100 — 450 10.0, Polymeric Silica Spher. 5 100 — 450 0 ODS-PREP Spher. 10 100 — 350 18.0, Monomeric

Calculated Bonded Phase Coverage (µmole/m2) — 3.50 5.00 — — — 3.01

End Capping No Yes Yes Yes No No Yes

Prodigy™ Phenyl-3

Prodigy Phenyl PH-3 A Polar Phenyl-Ethyl Column • Polymerically bonded phenyl-ethyl phase • Excellent for aromatic, polar compounds as well as difficult pharmaceutical drug separations

Synergi™ Polar-RP™

• Excellent option for applications requiring a USP L11 column

Increasing Hydrophobicity

• Different selectivity for many applications versus Luna Phenyl Hexyl and Synergi Polar-RP

Prodigy PH-3 is a phenyl phase that combines high purity B-type silica, polymeric bonding, moderate surface coverage as well as an ethyl-linker to provide a unique selectivity for a phenyl phase. Prodigy PH-3 is less hydrophobic than Luna Phenyl-Hexyl and Synergi Polar-RP due in part to differences in carbon load, linker, and endcapping. These differences make Prodigy PH-3 a specific option for applications requiring a phenyl phase: separations that can use π-π interactions between analytes and bonded phase to enhance aromatic selectivity differences. Typical applications where Prodigy PH-3 excels include polar aromatics as well as mixtures of polar and non-polar compounds.

Aq

Aq

Luna® Phenyl-Hexyl

Phenomenex Solutions for Phenyl Phase Selectivity The ability to participate in π-π interactions gives Phenomenex phenyl phases selectivities not available from conventional C8 or C18 columns. Three different phenyl phases are available, each offering unique phase characteristics: Prodigy Phenyl-3 - a polymerically bonded ethyl-linked phenyl phase with the lowest hydrophobicity of the phenyl phases. Low hydrophobicity and no additional polar functionality result in aromatic interactions being most focused with this phase. Synergi Polar-RP - an ether-linked phenyl phase with polar endcapping that has moderate hydrophobicity. By having polar endcapping and a polar embedded group Synergi Polar-RP has the most polar selectivity of the phenyl phases. (see p. 237) Luna Phenyl-Hexyl - a phenyl column with a hexyl linker and non-polar endcapping results in a phenyl phase with significant hydrophobicity. Such high hydrophobicity results in a phenyl phase with strong aromatic and hydrophobic selectivity. (see p. 158)

206

Using a Phenyl Phase Phenyl phases can provide separations not achievable on C18 or C8; such as increased retention for polar, aromatic compounds as well as reversals in analyte elution order. The unique selectivity is due to the interactions between the π electrons of the phenyl ring and π electrons from aromatic groups of the analytes. To fully express a phenyl’s unique aromatic selectivity, methanol should be used as the organic component rather than acetonitrile. It is presumed the π electrons of the “CN” bond in acetonitrile compete for the phenyl “binding sites” on the stationary phase, thus limiting the amount of π-π interaction between the stationary phase and the analyte molecules, and the separation would then be based only on hydrophobic retention. Synergi is a trademark of Phenomenex, Inc. Luna is a registered trademark of Phenomenex, Inc.

Phenomenex

PRODIGY™

App ID 10247

Phenyl PH-3 (continued) Polar Stationary Phase Selectivity Columns: Dimensions: Mobile Phase: Flow Rate: Detection: Sample:

Prodigy PH-3 Conventional C18 column 150 x 4.6 mm Acetonitrile/Water 45:55 for Prodigy PH-3, 65:35 for conventional C18 1.0 mL/min UV @ 254 nm 1. Ethylbenzene 2. Naphthalene

HPLC

vs. C18

Prodigy PH-3

Column: Prodigy 5 μm PH-3 Dimensions: 150 x 4.6 mm 00F-4298-E0 Part No.: Mobile Phase: Acetonitrile / 20 mM Potassium phosphate pH 3.0 (25:75) Flow Rate: 1.5 mL/min Temperature: 40 °C Detection: 0 - 1.6 min UV @ 230 nm 1.6 - 2 min UV @ 210 nm 2 min UV @ 230 nm Sample: 1. Saccharin 2. Aspartame 3. p-Hydroxybenzoic Acid 4. Caffeine 5. Benzoic Acid 6. p-Hydroxybenzoic Acid Methyl Ester 7. p-Toluic Acid 8. Dehydroacetic Acid 9. p-Hydroxybenzoic Acid Ethyl Ester 10. p-Hydroxybenzoic Acid Propyl Ester

App ID 10251

App ID 10257

Prodigy

Food Additives

Pseudoephedrine, Chlorpheniramine, and Dextromethorphan in Cough and Cold Formulations Column: Dimensions: Part No.: Mobile Phase: Flow Rate: Temperature: Detection: Sample:

Prodigy 5 μm PH-3 150 x 4.6 mm 00F-4298-E0 Methanol / Water (60:40) containing 2.5 mM Monobasic potassium phosphate, 3 mM Triethylamine, 0.5 mM Sodium lauryl sulfate, and 0.1 % Phosphoric acid 2.0 mL/min 25 °C UV @ 214 nm 1. Acetaminophen 2. Pseudoephedrine (USP tailing factor 1.67) 3. Chlorpheniramine (USP tailing factor 1.41, Rs from peak 2 = 10.68) 4. Dextromethorphan (USP tailing factor 1.31, Rs from peak 3 = 6.51)

8

1 3

5

1

7

2

4

6 9

0

Phenomenex

2

4

6

10 8

10

2 12

14 min

0

3 2

4 4

6 min

207

PRODIGY™ ODS and C8 Low Silanol Activity

Closeness of the two peaks indicates less silanol activity.

Conditions for All Columns Dimensions: Mobile Phase: Flow Rate: Detection: Sample:

150 x 4.6 mm Hexane 1 mL/min UV @ 254 nm 1. Benzene 2. o-Nitroanisole

mAU

2

2

400

800

300

600

1

100

0

400

App ID 5466

200

App ID 5464

1

App ID 5462

Prodigy

HPLC

This inert test, performed in normal phase mode, is sensitive to the presence of free silanols. When residual silanol groups are present, o-nitroanisole elution is delayed. Increased retention, i.e., delayed elution of o-nitroanisole with respect to benzene, indicates adsorption of its methoxy group to residual free silanols on the bonded phase.

200

0 5 min

Prodigy 5 μm C8

0

Prodigy 5 μm ODS (2)

2

4

min

Prodigy 5 μm ODS (3)

Excellent Peak Asymmetry Conditions for All Columns

208

App ID 5455

App ID 5457

150 x 4.6 mm Acetonitrile/Water (65:35) 1. 0 mL/min UV @ 254 nm 1. Acetophenone 2. Benzene 3. Toluene 4. Naphthalene

App ID 5456

Dimensions: Mobile Phase: Flow Rate: Detection: Sample:

Prodigy 5 μm C8 Peak Asymmetry =1.05

Prodigy 5 μm ODS (2) Peak Asymmetry =1.01

Prodigy 5 μm ODS (3) Peak Asymmetry =1.06

Phenomenex

PRODIGY™ ORDERING INFORMATION

SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

3 μm ODS-3 Columns (mm) SecurityGuard™Cartridges 100 x 2.0 100 x 4.0 30 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 mm* 4 x 3.0 mm* Phases /10pk /10pk ODS-3 100 Å I 00D-4222-B0 I 00D-4222-D0 I 00A-4222-E0 I 00C-4222-E0 I 00D-4222-E0 I 00F-4222-E0 AJ0-4286 I AJ0-4287 for ID: 2.0-3.0 mm

PEEK columns available upon request.

3.2-8.0 mm

00F-4243-E0

150 x 4.6

5 μm ODS-3V

00F-4241-E0

150 x 4.6

ODS-2 150 Å



00F-3300-A0

5 μm ODS-3V

00G-4241-E0

250 x 4.6

ODS-3 100 Å

00B-4097-A0

00F-4097-A0

Columns (mm) 50 x 2.0 I 00B-3301-B0

I



100 x 2.0 —

Price

I





150 x 1.0

I

00F-3301-A0



SecurityGuard™Cartridges 250 x 2.0 4 x 2.0 mm* /10pk I — AJ0-4289

150 x 2.0 00F-3301-B0



ODS-2 150 Å



00D-3300-B0

00F-3300-B0



AJ0-4286

ODS-3 100 Å

00B-4097-B0

00D-4097-B0

00F-4097-B0

00G-4097-B0

AJ0-4286

Phenyl-3 (PH-3) 100 Å

00B-4298-B0

00D-4298-B0

00F-4298-B0

00G-4298-B0

Prodigy

5 μm Minibore Phases C8 150 Å

I

HPLC

3 μm ODS-3V

5 μm Microbore Columns (mm) 50 x 1.0 Phases I C8 150 Å —

3 μm and 5 μm ODS-3V Columns (mm) Phases Part No. Size (mm) I I 3 μm ODS-3V 00D-4243-E0 100 x 4.6

AJ0-4350 for ID: 2.0-3.0 mm

5 μm MidBore™ Columns (mm) 150 x 3.0 250 x 3.0 Phases I C8 150 Å 00F-3301-Y0 I 00G-3301-Y0 ODS-2 150 Å



100 x 3.2 I —

— 00G-3300-Y0

I

150 x 3.2 —



SecurityGuard™Cartridges 4 x 2.0 mm* 4 x 3.0 mm* /10pk /10pk I AJ0-4289 AJ0-4290



250 x 3.2 I 00G-3301-R0



00F-3300-R0

00G-3300-R0

AJ0-4286

AJ0-4287

ODS-3 100 Å

00F-4097-Y0 00G-4097-Y0 00D-4097-R0

00F-4097-R0

00G-4097-R0

AJ0-4286

AJ0-4287

Phenyl-3 (PH-3) 100 Å

00F-4298-Y0 00G-4298-Y0

AJ0-4350

AJ0-4351







for ID: 2.0-3.0 mm

5 μm and 10 μm Analytical Columns (mm) 30 x 4.6 50 x 4.6 Phases I I 5 μm Silica 150 Å — —

3.2-8.0 mm

SecurityGuard Cartridges 250 x 4.6 4 x 3.0 mm* /10pk I 00G-3302-E0 AJ0-4348 ™



100 x 4.6 I —

I

150 x 4.6 —





5 μm C8 150 Å 00A-3301-E0 00B-3301-E0

00D-3301-E0

00F-3301-E0

00G-3301-E0

AJ0-4290

5 μm ODS-2 150 Å 00A-3300-E0 00B-3300-E0

00D-3300-E0

00F-3300-E0

00G-3300-E0

AJ0-4287

5 μm Silica 100 Å

— 00B-4098-E0

00D-4098-E0



00G-4098-E0

AJ0-4348

5 μm ODS-3 100 Å 00A-4097-E0 00B-4097-E0

00D-4097-E0

00F-4097-E0

00G-4097-E0

AJ0-4287

5 μm Phenyl-3 (PH-3) 100 Å 00A-4298-E0 00B-4298-E0

00D-4298-E0

00F-4298-E0

00G-4298-E0

AJ0-4351 AJ0-4348

10 μm Silica-3 100 Å









00G-4245-E0

10 μm ODS-3 100 Å









00G-4244-E0

AJ0-4287 for ID: 3.2-8.0 mm

5 μm and 10 μm Semi-Preparative and Preparative Columns (mm) 250 x 10 250 x 21.2 250 x 50 Phases I I I 5 μm ODS-3 100 Å 00G-4097-N0 00G-4097-P0 — 5 μm Phenyl-3 100 Å

00G-4298-N0



10 μm ODS-3 100 Å

00G-4244-N0

00G-4244-P0

SecurityGuard™Cartridges 10 x 10 mm‡ 15 x 21.2 mm** /3pk /ea I AJ0-7839 AJ0-7221



AJ0-7314

AJ0-7841

00G-4244-V0

AJ0-7221

AJ0-7839



10 μm-PREP Columns (mm) 250 x 4.6 250 x 10 250 x 21.2 Phases I 00G-4088-E0 I 00G-4088-N0 I 00G-4088-P0 ODS-PREP



250 x 50 I 00G-4088-V0

for ID: 9-16 mm

18-30 mm

Guards SecurityGuard™Cartridges 50 x 50 4 x 3.0 mm* 10 x 10 mm‡ 15 x 21.2 mm** /10pk /3pk ea I AJ0-7221 I AJ0-7839 03B-4088-VO AJ0-4287 for ID: 3.2-8.0 mm

9-16 mm

18-30 mm

*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282 Semi-prep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220 **PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223



See p. 218 for SecurityGuard Cartridge Holders.

Phenomenex

209