Human Blood Group Rh D (ISBT No. 4001) / CD240D
Human IgM, Lambda light chain
Antigen Description and Distribution The Rh D antigen (Rh1 or Rho) is clinically the most important of the Rh blood group system. It is expressed on the extracellular loops of a transmembrane polypeptide of around Mr 300001. In humans the Rh D antigen is expressed solely on the erythrocytes of Rh D positive individuals. 85% of Caucasians are Rh D positive. Clone The cell line producing RUM 1 is a human heterohybridoma derived from the fusion of EBV transformed B cells, from the peripheral blood of a donor producing high levels of anti-D, with X63Ag8.653 myeloma cells2. RUM 1 reacts as a direct agglutinin with all Rh D positive red cells except those of DVI type confirming the designated epitope as epD6/7h3,4,5. RUM 1 reacts with HMi, HMii, DFR, DBT and RoHAr Rh D types3. RUM 1 (code I-103) was submitted to the third international workshop on monoclonal antibodies against human red cells, Nantes 19966. RUM 1 can be used as a reagent both for manual tests and in automated cell groupers for the reliable detection of Du7. References 1. Cartron, J-P, (1994) Blood Reviews 8, 199-212. 2. Melamed et al (1987) J. Immunol. Methods, 104, 245-251. 3. Jones J. et al, (1996) Vox Sang 71, 176-183. 4. Lloyd-Evans et al (1999) British J. Immunol. 104, 621-625. 5. Avent et al (1997) Blood 89, 1779-1786. 6. Rouger P Muller JY (Eds) (1997) Proceedings of the third International workshop and symposium on monoclonal antibodies against human red cells and related antigens, Nantes 1996. 7. Scott et al (1993) Transfusion 33, poster presented at AABB meeting October 1993.